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Allen Cell Collection
AICS-0010
Protein
Sec61 beta
Gene Symbol
SEC61B
Gene Name
SEC61 translocon beta subunit
Structure
endoplasmic reticulum
Certificate of AnalysishPSCreg Certificate

Obtain AICS-0010

Obtain Donor Plasmid

AICS-0010

SEC61B in WTC-mEGFP (mono-allelic tag)
Cell line media

hiPS cell colony expressing mEGFP tagged Sec61-beta imaged on a spinning-disk confocal microscope. Left panel is a single slice near the bottom of the cell; right panel is a single slice near the middle of the cell.

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NCBI Isoforms:NM_006808
crRNA Target Site:GCCATACCATATTGGAGATG
Linker: SGLRS
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: SEC61B locus; Bottom: Zoom in on mEGFP insertion site at SEC61B N-terminal exon