AICS-0012
Protein
alpha-tubulin
Gene Symbol
TUBA1B
Gene Name
tubulin-alpha 1b
Structure
microtubules

Obtain AICS-0012

Obtain Donor Plasmid

AICS-0012

TUBA1B in WTC-mEGFP (mono-allelic tag)
Cell line media

Single image of a live hiPSC colony expressing alpha-tubulin-mEGFP imaged on a spinning-disk confocal microscope. Image is a single slice taken in the middle of the z-stack.

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NCBI Isoforms:NM_006082
crRNA Target Site:GATGCACTCACGCTGCGGGA
Linker: GGSGGS
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: TUBA1B locus; Bottom: Zoom in on mEGFP insertion site at TUBA1B N-terminus