hiPS cells expressing mEGFP-tagged outer mitochondrial membrane receptor Tom20 (TOMM20) and mTagRFP-T-tagged mitochondrial targeting sequence of COXVIII (mitoTag). Image is a single slice near the bottom of the cell (scalebar, 5 μm). Inset image is 2x enlargement of boxed area to show detailed relationship between tagged proteins. Cells were imaged live in 3D on a spinning-disk confocal microscope.