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Allen Cell Collection
AICS-0016
Protein
beta-actin
Gene Symbol
ACTB
Gene Name
actin beta
Structure
actin filaments
Certificate of AnalysishPSCreg Certificate

Obtain AICS-0016

Obtain Donor Plasmid

AICS-0016

ACTB in WTC-mEGFP (mono-allelic tag)
Cell line media

Single images of a live hiPS cell colony expressing mEGFP tagged beta-actin imaged on a spinning-disk confocal microscope. Left panel is a single slice near the bottom of the cell; right panel is a single slice near the top of the cell

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NCBI Isoforms:NM_001101
crRNA Target Site:GCCGTTGTCGACGACGAGCG
Linker: AGSGT
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: ACTB locus; Bottom: Zoom in on mEGFP insertion site at ACTB N-terminal exon