AICS-0017
Protein
desmoplakin
Gene Symbol
DSP
Gene Name
desmoplakin
Structure
desmosomes

Obtain AICS-0017

Obtain Donor Plasmid

AICS-0017

DSP in WTC-mEGFP (mono-allelic tag)
Cell line media

Single image of a live hiPSC colony expressing Desmoplakin-mEGFP imaged on a spinning-disk confocal microscope. Left panel is a maximum intensity projection of the z-stack; right panel is this same projection overlaid onto one slice of the same z-stack in the transmitted light channel.

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NCBI Isoforms:NM_004415
crRNA Target Site:AGAACTACTGCTAAATGAGT
Linker: HDPPVAT
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: DSP locus showing 3 DSP isoforms; Bottom: Zoom in on mEGFP insertion site at DSP C-terminal exon