AICS-0023
Protein
tight junction protein ZO-1
Gene Symbol
TJP1
Gene Name
tight junction protein 1
Structure
tight junctions

Obtain AICS-0023

Obtain Donor Plasmid

AICS-0023

TJP1 in WTC-mEGFP (mono-allelic tag)
Cell line media

Single image of a live hiPS cell colony expressing mEGFP tagged tight junction protein ZO-1 imaged on a spinning-disk confocal microscope. Image is a maximum intensity projection of a 3D z-stack.

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NCBI Isoforms:NM_003257.4
crRNA Target Site:GGCCGCGGAGGCGCTCACCT
Linker: SGLRSRALERDK
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: TJP1 locus showing 4 TJP1 isoforms; Bottom: Zoom in on mEGFP insertion site at TJP1 N-terminal exon