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Allen Cell Collection
AICS-0024
Protein
non-muscle myosin heavy chain IIB
Gene Symbol
MYH10
Gene Name
myosin heavy chain 10
Structure
actomyosin bundles
Certificate of AnalysishPSCreg Certificate

Obtain AICS-0024

Obtain Donor Plasmid

AICS-0024

MYH10 in WTC-mEGFP (mono-allelic tag)
Cell line media

Single images of a live hiPS cell colony expressing mEGFP tagged non-muscle myosin heavy chain IIB imaged on a spinning-disk confocal microscope. Left panel is a single slice near the bottom of the cell; right panel is a single slice near the top of the cell.

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NCBI Isoforms:NM_001256012
crRNA Target Site:GTTCTCTGCGCCATTGTAAA
Linker: YSDLELKLRIP
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: MYH10 locus showing 3 MYH10 isoforms; Bottom: Zoom in on mEGFP insertion site at MYH10 N-terminal exon