mEGFP-tagged autophagy-related protein LC3 B localization in an hiPSC colony. Image is a single slice of a 3Dspinning disk confocal z-stack of a live hiPSC colony in the bottom half of the cells. Both untreated cells (left panel) andcells treated for 24 hours with 800nM leupeptin to visualize autophagosomes (right panel) are shown.