Left: Single, mid-level plane of cells in a live hiPS cell colony expressing mEGFP-tagged SERCA2 protein. Cells were imaged four days after plating on Matrigel-coated glass as described in methods at www.allencell.org. Scale bar, 5 µm. Right: Single, near-bottom plane of hiPSC-derived cardiomyocytes expressing mEGFP-tagged SERCA2 protein.Twelve days after the onset of differentiation, cells were plated on PEI and laminin coated glass and imaged 29 days later (41 days total after the onset of differentiation). Inset shows detail of SERCA2 in longitudinal sarcoplasmic reticulum (SR). Scale bars, 10 µm. Cells were imaged in 3D on a spinning-disk confocal microscope.