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Allen Cell Collection
AICS-0054
Protein
CAAX domain of K-Ras
Gene Symbol
AAVS1
Gene Name
safe harbor locus
Structure
plasma membrane
Certificate of AnalysishPSCreg Certificate

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AICS-0054

AAVS1 in WTC-mTagRFP-T (mono-allelic tag)
Cell line media

Single-planes of live hiPS cell colony expressing the CAAX domain of K-Ras tagged with mEGFP expressed from a safe harbor locus. Z-planes shown are at the bottom (left), middle (middle) and top (right) of cells. Cells were imaged in 3D on a spinning-disk confocal microscope. Scale bar, 5µm.

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NCBI Isoforms:N
crRNA Target Site:GGGGCCACTAGGGACAGGAT
Linker: NA
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: mTagRFPT insertion site at safe harbor locus (AAVS1) in PPP1R12C intron; Bottom: Zoom in on mTagRFPT insertion site at safe harbor locus (AAVS1); insertion into AAVS1 is based on Hockmeyer et al (2011) Nat. Biotechnology, 29(8): 731-734