AICS-0058
Protein
beta-catenin
Gene Symbol
CTNNB1
Gene Name
catenin beta 1
Structure
adherens junctions

Obtain AICS-0058

Obtain Donor Plasmid

AICS-0058

CTNNB1 in WTC-mEGFP (mono-allelic tag)
Cell line media

Single-planes of live hiPS cell colony expressing mEGFP-tagged-beta-catenin. Z-planes shown are at the bottom (left), middle (middle) and top (right) of cells. Cells were imaged in 3D on a spinning-disk confocal microscope. Scale bar, 5µm.

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NCBI Isoforms:n
crRNA Target Site:TGAAAATCCAGCGTGGACAA
Linker: SGLRSRAQASNSAVDGTAAT
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: CTNNB1 locus showing 4 CTNNB1 isoforms; Bottom: Zoom in on mEGFP insertion site at CTNNB1 N-terminal exon