Single, mid-level plane of cells in a live hiPS cell colony expressing mEGFP-tagged Sec61 beta and mTagRFP-T-tagged lamin B1. Panels show individual channels for Sec61 beta (left) and lamin B1 (middle) and the overlay of the two (right). Imaged region captures cells at different stages of the cell cycle to highlight cell cycle-dependent variation in co-localization of Sec61 beta and Lamin B1. Cells were imaged in 3D on a spinning-disk confocal microscope. Scale bar, 5 µm.