AICS-0069
Protein
nucleoporin Nup153
Gene Symbol
NUP153
Gene Name
nucleoporin 153
Structure
nuclear pores

Obtain AICS-0069

Obtain Donor Plasmid

AICS-0069

NUP153 in WTC-mEGFP (mono-allelic tag)
Cell line media

Single, mid-level plane of cells in a live hiPS cell colony expressing mEGFP-tagged Nucleoporin Nup153 protein. Cells were imaged in 3D on a spinning-disk confocal microscope. Inset depicts the bottom z-section of the boxed cell to show localization of Nucleoporin Nup153 protein across the surface of the nuclear envelope. Scale bar, 5 µm.

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NCBI Isoforms:n
crRNA Target Site:GCCATGGCCTCGGGAGCCGG
Linker: YSDLE
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: NUP153 locus showing 3 NUP153 isoforms; Bottom: Zoom in on mEGFP insertion site at NUP153 N-terminal exon