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Allen Cell Collection
AICS-0078
Protein
Tom20alpha-tubulin
Gene Symbol
TOMM20TUBA1B
Gene Name
translocase of outer mitochondrial membrane 20tubulin-alpha 1b
Structure
mitochondriamicrotubules
Certificate of AnalysishPSCreg Certificate

Obtain AICS-0078

Obtain Donor Plasmid

AICS-0078

TOMM20 in WTC-mEGFP (mono-allelic tag)
Cell line media

Single, mid-level plane of cells in a live hiPS cell colony expressing mEGFP-tagged Tom20 and mTagRFP-T-tagged alpha-tubulin. Panels show individual channels for Tom20 (left), alpha-tubulin (middle), and the overlay of the two (right). Cells were imaged in 3D on a spinning-disk confocal microscope. Scale bar, 5μm.

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NCBI Isoforms:
crRNA Target Site:AATTGTAAGTGCTCAGAGCT / GATGCACTCACGCTGCGGGA
Linker: GGSGDPPVAT / GGSGGS
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: TOMM20 locus with zoom in on mEGFP insertion site at TOMM20 C-terminal exon. Bottom: TUBA1B locus with zoom in on mTagRFP-T insertion site at TUBA1B N-terminus.