AICS-0080
Protein
RNA-binding protein FUS
Gene Symbol
FUS
Gene Name
FUS RNA binding protein
Structure
paraspeckles / stress granules

Obtain AICS-0080

Obtain Donor Plasmid

AICS-0080

FUS in WTC-mEGFP (mono-allelic tag)
Cell line media

Single, mid-level plane of cells in a live hiPS cell colony expressing mEGFP-tagged RNA-binding protein FUS in control cells (left panel) and in the presence of 500 µM sodium arsenite for 15 min (right panel). Cells were imaged in 3D on a spinning-disk confocal microscope. Scale bar, 5 µm.

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NCBI Isoforms:n
crRNA Target Site:ACAGACAGGATCGCAGGGAG
Linker: ENLYFQGAAKFKETAAAKFERQHMDSGGGGSSGPSGSSSLEVLFQGPLSSSGPSGS
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: FUS locus showing 3 FUS isoforms; Bottom: Zoom in on mEGFP insertion site at FUS C-terminal exon