AICS-0082
Protein
Ras GTPase-activating protein-binding protein 1 (G3BP-1)
Gene Symbol
G3BP1
Gene Name
G3BP stress granule assembly factor 1
Structure
stress granules

Obtain AICS-0082

Obtain Donor Plasmid

AICS-0082

G3BP1 in WTC-mEGFP (mono-allelic tag)
Cell line media

hiPS cells expressing mEGFP-tagged Ras GTPase-activating protein-binding protein 1 (G3BP-1) in control cells (left panel; single mid-level plane) and in cells treated with 62.5 μM sodium arsenite for 60 minutes (right panel; maximum intensity z-projection). Cells were imaged live in 3D on a spinning-disk confocal microscope. Scale bar, 5 μm.

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NCBI Isoforms:
crRNA Target Site:TTGCTTTGGTCAATTCAACC
Linker: SGLRS
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: G3BP1 locus showing 2 G3BP1 isoforms; Bottom: Zoom in on mEGFP insertion site at G3BP1 N-terminal exon.