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Allen Cell Collection
AICS-0086
Protein
fibrillarinnucleophosminnucleolar transcription factor UBF
Gene Symbol
FBLNPM1UBTF
Gene Name
fibrillarinnucleophosmin 1upstream binding transcription factor
Structure
nucleolus (dense fibrillar component)nucleolus (granular component)nucleolus (fibrillar center)
Certificate of AnalysishPSCreg Certificate

Obtain AICS-0086

Obtain Donor Plasmid

AICS-0086

FBL in WTC-mEGFP (mono-allelic tag)
Cell line media

Single, mid-level plane of cells in a live hiPS cell colony expressing mEGFP-tagged fibrillarin, mTagRFP-T-tagged nucleophosmin, and HaloTag-tagged nucleolar transcription factor UBF visualized with ligand Janelia Fluor 646 (Promega). Panels show individual channels for fibrillarin, nucleolar transcription factor UBF, nucleophosmin, and an overlay of the three (counterclockwise from top right). Cells were imaged in 3D on a spinning-disk confocal microscope. Scale bar, 5μm.

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NCBI Isoforms:n
crRNA Target Site:AACTGAAGTTCAGCGCTGTC / TCCAGGCTATTCAAGATCTC / CGAGGAGGTGGCTGGACAGC
Linker: KPNSAVDGTAGPGSIAT / KPNSAVDGTAGPGSIAT / SG
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: FBL locus with zoom in on mEGFP insertion site at FBL C-terminal exon. Middle: NPM1 locus showing 7 NPM1 isoforms with zoom in on mTagRFP-T insertion site at NPM1 C-terminal exon. Bottom: UBTF locus showing 3 UBTF isoforms with zoom in on HaloTag insertion site at UBTF N-terminal exon