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Allen Cell Collection
AICS-0089
Protein
fibrillarinnucleophosmindCas9-KRAB
Gene Symbol
FBLNPM1CLYBL-dCas9-KRAB
Gene Name
fibrillarinnucleophosmin 1citramalyl-CoA lyase safe harbor
Structure
nucleolus (dense fibrillar component)nucleolus (granular component)nucleolus (nucleus)
Certificate of AnalysishPSCreg Certificate

Obtain AICS-0089

Obtain Donor Plasmid

AICS-0089

FBL in WTC-mEGFP (mono-allelic tag)
Cell line media

Single, mid-level plane of cells in a live hiPS cell colony expressing mEGFP-tagged fibrillarin, mTagRFP-T-taggednucleophosmin, and TagBFP-tagged dCas9-KRAB. Panels show individual channels for fibrillarin, nucleophosmin, the overlayof the two, and dCas9-KRAB (clockwise from the top left). Cells were imaged in 3D on a spinning-disk confocal microscope.Scale bar, 5μm.

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NCBI Isoforms:n
crRNA Target Site:AACTGAAGTTCAGCGCTGTC / TCCAGGCTATTCAAGATCTC / TGTTGGAAGGATGAGGAAAT
Linker: KPNSAVDGTAGPGSIAT / KPNSAVDGTAGPGSIAT
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: FBL locus with zoom in on mEGFP insertion site at FBL C-terminal exon. Middle: NPM1 locus showing 7 NPM1 isoforms with zoom in on mTagRFP-T insertion site at NPM1 C-terminal exon. Bottom: CLYBL locus showing dCas9-TagBFP-KRAB insertion site at CLYBL safe harbor site between exons 2 and 3.