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Allen Cell Collection
AICS-0090
Protein
dCas9-KRAB
Gene Symbol
CLYBL safe harbor
Gene Name
citramalyl-CoA lyase safe harbor
Structure
nucleus
Certificate of AnalysishPSCreg Certificate

Obtain AICS-0090

Obtain Donor Plasmid

AICS-0090

CLYBL safe harbor in WTC-TagBFP (mono-allelic tag)
Cell line media

Single, mid-level plane of cells in a live hiPS cell colony expressing TagBFP-tagged dCas9-KRAB. TagBFP-tagged dCas9-KRAB (left) and overlay onto transmitted light image (right) are shown. Cells were imaged in 3D on a spinning-disk confocal microscope. Scale bar, 50 μm.

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NCBI Isoforms:n
crRNA Target Site:TGTTGGAAGGATGAGGAAAT
Linker: N/A
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: CLYBL locus; Bottom: Zoom in on dCas9-TagBFP-KRAB insertion site at CLYBL safe harbor site between exons 2 and 3