AICS-0096
Protein
RPB1
Gene Symbol
POLR2A
Gene Name
RNA polymerase II subunit A
Structure
RNA polymerase II

Obtain AICS-0096

Obtain Donor Plasmid

AICS-0096

POLR2A in WTC-mEGFP (mono-allelic tag)
Cell line media

Single, mid-level plane of cells in a live hiPS cell colony expressing mEGFP-tagged RPB1. Cells were imaged in 3D on a spinning-disk confocal microscope. Scale bar, 50μm

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NCBI Isoforms:n
crRNA Target Site:CGCCTGCCTCCGCCATGCAC
Linker: SGLRSRAQASNSAVDGTAGPGSTGSS
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: POLR2A locus; Bottom: Zoom in on mEGFP insertion site at POLR2A N-terminal exon