AICS-0099
Protein
CCCTC-binding factor (CTCF)
Gene Symbol
CTCF
Gene Name
CCCTC-binding factor
Structure
CTCF-binding sites

Obtain AICS-0099

Obtain Donor Plasmid

AICS-0099

CTCF in WTC-mEGFP (mono-allelic tag)
Cell line media

Single, mid-level plane of cells in a live hiPS cell colony expressing mEGFP-tagged CCCTC-binding factor (CTCF). Cells were imaged in 3D on a spinning-disk confocal microscope. Scale bar, 5µm.

thumbnail image
thumbnail image
Video thumbnail
Video thumbnail
Video thumbnail
NCBI Isoforms:
crRNA Target Site:TGATGCAGTCGAAGCCATTG
Linker: EDLYFQS
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: CTCF locus showing 2 CTCF isoforms; Bottom: Zoom in on mEGFP insertion site at CTCF N-terminal exon