AICS-0104
SNPNM_000257.4(MYH7):c.752C>A(p.His251Asn)
Gene SymbolMYH7
Gene Namemyosin heavy chain 7
Parental LineAICS-0075 cl. 85 ACTN2
Clone NumberClone TypeReplicateGenotype
3MutantAH251N/WT
4ControlAWT/WT
6ControlAWT/WT
85MutantBH251N/WT
Certificate of Analysis

Obtain AICS-0104

2 mutant clones2 isogenic controls

AICS-0104

MYH7 in WTC-mEGFP-ACTN2 (mono-allelic tag)
Representative images for all clones
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One vial of distribution lot was thawed (cells were treated with ROCK inhibitor for 24hrs post-thaw - refer to culture protocol). A six panel image of clone 85 (H251N/wt) is shown here and is a representative image for all clones in the collection (except clone 4). Cultures were observed daily. Colonies were imaged one (a,b), four (c,d) and five (e,f) days post-thaw using a Leica microscope at 4x and 10x magnification. Scale bars are shown.

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cRNA Target Site:5’ ATTCATTCGAATTCATTTTG[GGG] 3’
DNA Donor Sequence:
Mutant* 5’ CCATCTCTCCAGGGGAAATTCATTCGAATT[A]ATTTTGGGGCAACAGGAAAGTTGGCATC 3’
Cas9:TrueCut™ Cas9 Protein
F Primer for PCR/Sequencing:5’ TCTCCTGATTTGAGGCTTGC 3’
R Primer for PCR/Sequencing:5’ AAAGACACCTAGCCATGCAG 3’
Red = PAM Site, Blue = Mutation
CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below.
CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below.
Top: MYH7 locus showing 1 MYH7 isoform; Bottom: Zoom in on mutation site at isoform NM_000257.4(MYH7):c.752C>A(p.His251Asn)
CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below.
CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below.
Top: ACTN2 locus showing 3 ACTN2 isoforms; Bottom: Zoom in on mEGFP insertion site at ACTN2 C-terminus