AICS-0105
SNPNM_000257.4(MYH7):c.1106G>A(p.Arg369Gln)
Gene SymbolMYH7
Gene Namemyosin heavy chain 7
Parental LineAICS-0075 cl. 85 ACTN2
Clone NumberClone TypeReplicateGenotype
22ControlAWT/WT
30MutantAR369Q/WT
31MutantAR369Q/R369Q
32MutantAR369Q/WT
57MutantBR369Q/WT
89ControlBWT/WT
Certificate of Analysis

Obtain AICS-0105

4 mutant clones2 isogenic controls

AICS-0105

MYH7 in WTC-mEGFP-ACTN2 (mono-allelic tag)
Representative images for all clones
main image

One vial of distribution lot was thawed (cells were treated with ROCK inhibitor for 24hrs post-thaw - refer to culture protocol). A four panel image of clone 57 (R369Q/wt) is shown here and is a representative image for all clones in the collection (except clone 89). Cultures were observed daily. Colonies were imaged one (D1) and four (D4) days post-thaw using a Leica microscope at 4x and 10x magnification. Scale bars are shown.

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cRNA Target Site: 5’ GTTCAAGCTGAAGCAGCGGG[AGG] 3’
DNA Donor Sequence:
Mutant* 5’ TGGAAACATGAAGTTCAAGCTGAAGCAGCAGGAGGAGC[A]GGCGGAGCCAGACGGCACTG 3’
Cas9:TrueCut™ Cas9 Protein
F Primer for PCR/Sequencing:5’ GCCAGGAAGCATAAGTGGGT 3’
R Primer for PCR/Sequencing:5’ GGTGACGTACTCATTGCCCA 3’
Red = PAM Site, Blue = Mutation
CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below.
CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below.
Top: MYH7 locus showing 1 MYH7 isoform; Bottom: Zoom in on mutation site at isoform NM_000257.4(MYH7):c.1106G>A(p.Arg369Gln)
CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below.
CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below.
Top: ACTN2 locus showing 3 ACTN2 isoforms; Bottom: Zoom in on mEGFP insertion site at ACTN2 C-terminus