AICS-0109
SNPNM_000257.4(MYH7):c.1573G>A (p.Glu525Lys)
Gene SymbolMYH7
Gene Namemyosin heavy chain 7
Parental LineAICS-0075 cl. 85 ACTN2
Clone NumberClone TypeReplicateGenotype
cl.55ControlBWT/WT
cl.56MutantBE525K/WT
cl.72ControlBWT/WT
cl.93MutantBE525K/WT
Certificate of Analysis

Obtain AICS-0109

2 mutant clones2 isogenic controls

AICS-0109

MYH7 in WTC-mEGFP-ACTN2 (mono-allelic tag)
Representative images for all clones
main image

One vial of distribution lot was thawed (cells were treated with ROCK inhibitor for 24hrs post-thaw - refer to culture protocol). Cultures were observed daily. Colonies were imaged one and four days post-thaw using a Leica microscope 4x and 10x magnification. A four panel image of clone 93 is shown here and is a representative image for all clones in the collection. Viability and colony formation one day and four days post-thaw. Scale bars are shown.

cRNA Target Site:5’ AAAGAGGCACCTTCTCGATG[AGG] 3’
DNA Donor Sequence:
5’ TCAGGTGGTAAGGCCAAAGAGGCACCTTCT[T]GATGAGGTCAATGC AGGCCTGCAGGTCC 3’
Cas9:TrueCut™ Cas9 Protein
F Primer for PCR/Sequencing: 5’ GGACTGTGTGGTGACAGAGG 3’
R Primer for PCR/Sequencing: 5’ GTGTGGGAGGTCATCATGCA 3’
Red = PAM Site, Blue = Mutation
HDR Editing Design: "Header Caption: CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below."
HDR Editing Design: "Header Caption: CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below."
Top: MYH7 locus showing 1 MYH7 isoform; Bottom: Zoom in on mutation site at isoform NM_000257.4(MYH7):c.1573G>A (p.Glu525Lys)
HDR Editing Design: "Header Caption: CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below."
HDR Editing Design: "Header Caption: CRISPR-Cas9 methodology was used to introduce a single base pair mutation to MYH7, and mEGFP at C-terminus of ACTN2 as shown below."
Top: ACTN2 locus showing 3 ACTN2 isoforms; Bottom: Zoom in on mEGFP insertion site at ACTN2 C-terminus